Sat. Jul 13th, 2024
"Multiphoton fluorescence image of HeLa cells" by National Institutes of Health (NIH) is licensed under CC BY-NC 2.0.

By Ivan Martinez, West Virginia University

In an amazing twist of fate, the aggressive cervical cancer tumor that killed Henrietta Lacks, a 31-year old African American mother, became an essential tool that helped the biomedical field flourish in the 20th century. As a cancer researcher who uses HeLa cells in my everyday work, even I sometimes find it hard to believe.

"Multiphoton fluorescence image of HeLa cells" by National Institutes of Health (NIH) is licensed under CC BY-NC 2.0.
“Multiphoton fluorescence image of HeLa cells” by National Institutes of Health (NIH) is licensed under CC BY-NC 2.0.

On Aug. 1, 2023, over 70 years after doctors took Lacks’ cells without her consent or knowledge, her family reached a settlement with biotech company Thermo Fisher. Lacks’ descendants had sued the company in 2021 for making billions of dollars off her cells. The family has not been previously been compensated.

Lacks’ cervical cancer cells, called “HeLa” after the first two letters of her first and last name, are immortal, continuing to divide when most cells would die. This ability to survive through endless generations of cells is what makes them invaluable for scientists conducting experiments on human cells.

Why HeLa cells matter

Before HeLa cells, scientists wanted a way to grow and study human cells in the lab to conduct studies that are impossible to do in a living person. When Lacks’ cervical cancer cells were successfully grown in a petri dish in 1951, scientists now had a source of cost-effective and easy-to-use cells that expanded their ability to conduct research. From polio and COVID-19 vaccines to cancer research and sequencing the human genome, HeLa cells have played an enormous role in many scientific discoveries and advancements.

Henrietta Lacks’ story is also an ongoing bioethics case, because these cells were taken from her during a routine cervical cancer biopsy and were then given to researchers without her consent, as was common practice at the time. The Lacks family has long attempted legal action against companies they say have unfairly benefited from Henrietta’s cells. A 2010 book by journalist Rebecca Skloot details how HeLa cells affected both science and the Lacks family.

The Lacks family wasn’t aware that Henrietta’s cells had been harvested until Rolling Stone magazine journalist Michael Rogers contacted them two decades after her death.

But how did Lacks’ cells become immortal?

Lacks didn’t know that cells in her cervix were infected with a virus that causes one of the most common sexually transmitted diseases: human papillomavirus, or HPV. There are more than 150 different types of HPVs, but only a small group are known to cause cervical cancer. In fact, 99.7% of cervical cancers are HPV positive. Fortunately, most people infected with high-risk HPVs are able to clear out the virus before it becomes cancerous. HPV vaccinations can prevent over 90% of HPV-related cancers. But 10% of people with HPV infections on their cervix develop cancer. Sadly, Henrietta was one of the unlucky ones.

Her misfortune has helped elucidate how HPV works. Since the Nobel Prize-winning 1976 discovery of HPV’s essential role in cervical cancer, many scientists, including me, have been investigating how HPV causes cancer.

While some types of HPV cause warts on the skin, certain high-risk ones can cause cancer.

Two proteins

It turns out that the virus’ cancer-causing ability is linked to two proteins it produces. These viral proteins can target and destroy two major human proteins that protect against cancer, p53 and retinoblastoma (Rb). P53 and Rb act as sentinels making sure cells don’t accumulate harmful genetic mutations and stop dividing after a set number of cycles. My research has focused on how HPV proteins interact with tumor-suppressing proteins in different types of human cells, including HeLa.

Most cells divide around 40 to 60 times before they become too old to function properly and are naturally killed off. But HPV can allow cells to divide forever, because they attack the sentinels keeping uncontrolled division in check. After Lacks was infected with HPV 18, the second-most-common high-risk type of the virus, her cervical cells lost the ability to produce these sentinels. Without growth checks in place, her cells were able to divide indefinitely and became “immortal” – living on to this day both in test tubes and the 70,000 studies they’ve made possible.

This article was updated to note the Lacks family’s settlement with Thermo Fisher on Aug. 1, 2023.The Conversation

Ivan Martinez, Associate Professor of Microbiology, Immunology and Cell Biology, West Virginia University

This article is republished from The Conversation under a Creative Commons license. Read the original article.